Description
Intended Uses: This sandwich kit is for the accurate quantitative detection of human G Protein-activated Inward Rectifier Potassium Channel 2 (also known as KCNJ6/GIRK2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human KCNJ6/GIRK2 antibody. KCNJ6/GIRK2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human KCNJ6/GIRK2 Antibody is added and binds to KCNJ6/GIRK2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated KCNJ6/GIRK2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human KCNJ6/GIRK2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm